顾建国 教授

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Dr. Jianguo Gu, professor of Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology,
Tohoku Pharmaceutical University. He received the MD degree from the Nantong Medical School, China in 1987. He
obtained the PhD degree in 1993 from Department of Biochemistry, Osaka U niversity Medical School. From 1993 to
1997, he worked at Osaka Medical Center for Maternal and Child Health as a researcher. He then joined in Dr. Kenneth
Yamada’s lab in NIDCR/NIH, USA, as a postdoctoral fellow from 1997 to 1999. In 1999-2002, he was appointed as an assistant professor at the Institute for Protein Research, Osaka University. He was then recruited to the Department of
Biochemistry, Osaka University Graduate School of Medicine in 2002, and was promoted to associate professor.
In 2006, he was appointed as a full professor in the current place. 2007-present, he is a visiting senior researcher in
RIKEN, Japan. 2010- 2015, he is a visiting Professor in Dalian institute of chemical physics, Chinese Academy of Sciences,
China
Dr. Gu’s group has been performing extensive studies on the importance of N-glycans in physiology and pathology, and demonstrated the N-glycans play important roles in cancer invasion and metastasis, cell growth, differentiation and nervous system. He has published 24 review papers and 83 original papers as following.
Review papers in English
1. The PTEN gene and integrin signaling in cancer. Tamura, M., Gu, J ., Tran, H., and Yamada, KM. J. Natl. Cancer Inst . 91:
1820-1828, 1999. (167)
2. Functional glycomics and evidence for gain- and loss-of-functions of target proteins for glycosyltransferases involved in N-glycan biosynthesis: their pivotal roles in growth and development, cancer metastasis and antibody therapy against cancer.
Taniguchi, N., Gu, J., Takahashi, M., Miyoshi, E. Proc. J apan Acad. , Ser. B, 80, 82-91, 2004.
3. Regulation of integrin functions by N-glycan. Gu, J. and Taniguchi, N. Glycoconj. J., 21, 9-15, 2004.
4. Signaling and Glycoproteins. Zhao, Y., Gu, J., and Taniguchi, N. Comprehensive Glycoscience, 4.14: 249-265, 2007
5. Decoding of Sugar Functions by Identifying Target Glycoproteins. Taniguchi, N., Miyoshi, E., Gu, J., Honke, K., and Matsumoto,
A. Current Opinion in Structural Biology , 16:561-6, 2006
6. From glycomics to functional glycomics of sugar chains: identification of target proteins with functional changes using gene
targeting mice and knock down cells of FUT8 as examples. Kondo, A., Li, W., Nakagawa, T., Nakano, M., Koyama, N., Wang, X.,
Gu, J., Miyoshi, E., and Taniguchi, N. Biochem. Biophy. Acta 1764:1881-9, 2006
7. Phenotype changes of Fut8 knockout mouse: core fucosylation is crucial for the function of growth factor receptors. Wang, X.,
Gu, J., Miyoshi, E., Honke, K. and Taniguchi, N. Methods Enzymol. 417: 11-22, 2006
8. Branched N-glycans regulate the biological functions of integrins and cadherins. Zhao, Y., Sato, Y., Isaji, T., Fukuda, T.,
Matsumoto, A., Miyoshi, E., Gu, J., Taniguchi, N. FEBS J. 275: 1939-48, 2008
9. Functional roles of N-glycans in cell signaling and cell adhesion in cancer. Zhao, Y., Takahashi, M., Gu, J., Miyoshi, E., Matsumoto, A., Kitazume, S. and Taniguchi, N. Cancer Sci. 99: 1304-10, 2008
10. Potential of N-glycan in cell adhesion and migration: As either a positive or negative regulator. Gu, J. and Taniguchi, N. Cell Adhesion & Migration 2:4. 1-3, 2008
11. N-glycans Regulate Integrin alpha5beta1Functions. Gu, J., Sato, Y. and Isaji, T. Experimental Glycoscience (Springer), 358-359, 2008
12. A mutual regulation between cell-cell adhesion and N-glycosylation: implication of the bisecting GlcNAc for biological functions. Gu, J., Sato, Y., Kariya, Y., Isaji, T., Taniguchi, N. and Fukuda, T. J. Proteome Res. 8:431-435, 2009
13. Importance of N-glycosylation on a5b1 integrin for its biological functions. Gu, J., Isaji, T., Sato, Y., Kariya, Y. and Fukuda, T. Biol. Pharm. Bull.32:780-5, 2009
14. Roles for laminin-332 and beta4 integrin in tumor progression. Kariya, Y., Kariya, Y. and Gu, J. Mini-Rev Med Chem. 9: 1284-91, 2009
15. Functional roles of the bisecting GlcNAc in integrin-mediated cell adhesion.Isaji, T., Kariya, Y. Xu, Q., Fukuda, T., Taniguchi, N. and Gu, J. Methods Enzymol. 480: 445-459, 2010
16. Modulation of E-cadherin function and dysfunction by N-glycosylation. Pinho, SS., Seruca, R., G?rtner, F., Yamaguchi, Y., Gu, J., Taniguchi, N. and Reis, CA. Cellular and Molecular Life Sciences 68: 1011-20, 2011
17. Potential roles of N-glycosylation in cell adhesion. Gu, J., Isaji, T., Xu, Q., Kariya, Y., Gu, W., Fukuda, T. and Du, Y. Glycoconj. J . 29: 599-607,2012
18. Laminin-332 and Integrins: Signaling Platform for Cell Adhesion and Migration and its Regulation by N-glycosylation. Kariya, Y., Kariya, Yand Gu, J . Laminins: Structure, Biological Activity and Role in Disease, Nova Science Publishers Inc., NY, Chapter
2, 1-23, 2013
19. Gastric cancer: adding glycosylation to the equation. Pinho, SS., Sandra Carvalho, S., Marcos-Pinto, R., Magalh?es, A., Oliveira, C., Gu, J., Dinis-Ribeiro, M., Carneiro, F., Seruca, R. and Reis, CA. Trends in Molecular Medicine 19: 664-76, 2013
20. Integrin α5β1 and its N-glycosylation. Jianguo Gu, Qinglei Hang, Tomohiko Fukuda, Tomoya Isaji. Glycoscience: Biology and Medicine Springer, P. 970-975, 2014
21. Fucosyltransferase 8 (α1,6-fucosyltransferase, FUT8), Ihara, H., Tsukamoto, H., Gu, J., Miyoshi, E., Taniguchi, N. and Ikeda, Y. Handbook of Glycosyltransferase and related genes Springer, P.581-596, 2014
22. β1,4- N-Acetylglucosaminyltransferase III (GnT-III, GlcNAc-T III), Ikeda, Y., Ihara, H., Tsukamoto, H., Gu, J. and Taniguchi, N. Handbook of Glycosyltransferase and related genes Springer, P.209-222, 2014
23. α1,6-Fucosyltransferase knockout mice and Schizophrenia-like phenotype. Wei Gu, Tomohiko Fukuda, and Jianguo Gu. Sugar Chains Springer Japan, P.267-280, 2015
24. Significance of β-Galactoside α2,6 Sialyltranferase 1 in Cancers. Jishun Lu and Jianguo Gu, Molecules, 20:7509-7527, 2015
Original papers in English
1. Glycosyltransferase assays using pyridylaminated acceptors: N-acetylglucosaminyl-transferase III, IV and V involving HPLC. Taniguchi, N., Nishikawa, A., Fujii, S., and Gu, J. Methods Enzymol . 179: 397-408, 1989.
2. Determination of N-acetylglucosaminyltransferase III, IV and V in normal and hepatoma tissues of rats. Nishikawa, A., Gu, J., Fujii, S., and Taniguchi, N. Biochem. Biophy. Acta10335: 313-318, 1990.
3.A lack of expression of beta1-6Nacetylglucosaminyltransferase involved in the biosynthesis of blood group I antigen in the liver of LEC rats. Gu, J., Nishikawa, A., Matsuura, N., Sugiyama, T., and Taniguchi, N. Jpn. J. Cancer Res. 83: 878-884, 1992.
4. Biosynthesis of blood group I and i antigen in rat tissues: identification of a novel beta1-6N-acetylglucosaminyltransferase. Gu, J., Nishikawa, A., Fujii, S., Gasa, S., and Taniguchi, N. J. Biol. Chem . 267: 2994-2999, 1992.
5. Total metabolic flow of glycosphingolipid biosynthesis is regulated by UDP-GlcNAc: lactosylceramide alpha1-3N-acetylglucosaminytransferase and CMP-NeuAc: Lactosylceramide alpha2-3 sialytransferase in human hematopoietic cell line HL-60 during differentiation. Nakamura, M., Tsunoda, A., Sakeo, K., Gu, J., Nishikawa, A., Taniguchi, N., and Saito, M. J. Biol. Chem .
267: 23507-23514, 1992.
6.Purification and characterization of UDP-N-acetylglucosamine: alpha-6-D-mannoside beta1-6N-acetylglucosaminyltransferase from a human lung cancer cell line. Gu, J., Nishikawa, A., Tsuruoka, N., Ohno, M., Yamaguchi, N., Kangawa, K., and Taniguchi, N.
J. Biochem . 113: 614-619, 1993.
7. N-acetylglucosaminyl-transferase III and V messenger RNA levels in LEC rats during hepatocarcinogenesis. Miyoshi, E., Nishikawa, A., Ihara, Y., Gu, J., Sugiyama, T., Hayashi, N., Fusamoto, H., Kamada, T, and Taniguchi, N. Cancer Res . 53: 3899-3902, 1993.
8.Electrospray ionization mass spectrometry of pyridylaminated oligosaccharide derivative: sensitivity and in-source fragmentation. Gu, J., Hiraga, T., and Wada, Y. Biol. Mass Spectrom. 23: 212-217, 1994.
9. Diagonosis of carbohydrate-deficient glycoprotein syndrome by matrix-assisted laser desoption time-of-flight mass spectrometry. Wada, Y., Gu, J., Okamoto, N., and Inui, K. Biol. Mass Spectrom . 23: 108-109, 1994.
10. cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltrnasferase V. Gu, J., Saito, H., Nishikawa, A., Ihara, Y., Soejima, H., Wada, Y., Sekiyama, C., Niikawa, N., and Taniguchi, N. Biochem. Biophys. Res. Commun. 198: 318-327, 1994.
11.Oligosaccharide structures of immunoglobulin G from two patients with carbohydrate-deficient glycoprotein syndrome. Gu, J., Kondo, A., Okamoto, N., and Wada, Y. Glycosyl. Dis. 1: 247-252, 1994.
12.Aberrant expression of decorin and biglycan genes in the carbohydrate-deficient glycoprotein syndrome. Gu, J., and Wada, Y. J. Biochem. 117: 1276-1279, 1995.
13. Organization of the human N-acetylglucosaminyl-transferase V gene. Saito, H., Gu, J., Nishikawa, A., Ihara, Y., Fujii, J., Kohgo, Y., and Taniguchi, N. Eur. J. Biochem . 233: 18-26, 1995.
14.Effect of exogenous decorin on cell morphology and attachment of decorin-deficient fibroblasts. Gu, J., and Wada, Y. J. Biochem. 119: 743-748, 1996.
15.The production and purification of functional decorin in a baculovirus system. Gu, J., Nakayama, Y., Nagai, K., and Wada, Y.
Biochem. Biophys. Res. Commun . 232: 91-95, 1997.
16. Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN. Tamura, M., Gu, J., Matsumoto, K., Aota, S.,
Parsons, R., amd Yamada, KM. Science . 280: 1614-1617, 1998. (1050)
17. Tumor suppressor PTEN inhibits Integrin- and growth factor-mediated MAP kinase signaling pathways. Gu, J., Tamura, M., and Yamada, KM. J. Cell Biol . 143:1375-1383, 1998. (338)
18. Tumor suppressor PTEN inhibition of cell invasion, migration, and growht: differential involvement of focal adhesion kinase and p130 cas.
Tamura, M., Gu, J ., Takino, T., and Yamada, KM. Cancer Res. 59:442-449, 1999. (365)
19. Mutation of Pten/Mmac1 in mice causes neoplasia in multiple organ systems. Podsypanina, K., Ellenson, LH., Nemes A., Gu, J ., Tamura, M.,
Yamada, KM, Cordon-Cardo, C., Catoretti, G., Fisher, P., and Parsons, R. Proc. Natl. Acad. Sci . 98: 1563-1568, 1999. (660)
20. PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix-dependent phosphatidylinositol 3-kinase/Akt cell survival pathway. Tamura, M., Gu, J ., Danen, E.H.J., Takino, T., and Yamada KM. J. Biol. Chem . 274: 20693-20703, 1999. (298)
21. Shc and FAK differentially regulate cell motility and directionality modulated by PTEN. Gu, J ., Tamura, M., Pankov, R., Danen, E.H.J.,
Takino, T., Matsumoto, K., and Yamada KM. J. Cell Biol. 146: 389-403, 1999. (371)
22. Activation of mitogen-activated protein kinases cascade results in phosphorylation of NF-M tail domains in transfected NIH 3T3 cells. Li, BS., Veeranna, Gu, J ., Grant, P., and Pant ,HC. Eur. J. Biochem. 262: 1-8, 1999. (51)
23. Integrin alpha1beat1-mediated activation of cyclin-dependent kinase 5 activity is involved in neurite outgrowth and human neurofilament protein H Lys-Ser-Pro tail domain phosphorylation. Li, BS., Zhang, l., Gu, J., Amin, ND., and Pant, HC. J. Neurosci. 20: 6055-6062, 2000. (90)
24. Laminin-10/11 and Fibronectin Differentially Regulate Integrin-dependent Rho and Rac Activition via p130 Cas/CrkII/DOCK180 Pathway. Gu, J., Sumida, Y., Sanzen, N., and Sekiguchi, K. J. Biol. Chem. 276: 27090-27097, 2001. (122)
25. Laminin-10/11 and Fibronectin Differentially Prevent Apoptosis Induced by Serum Removal via PI 3-kinase/Akt- and MEK1/ERK-dependent Pathways. Gu, J ., Fujibayashi, A., Yamada, KM., and Sekiguchi, K. J. Biol. Chem. 277: 19922-19928, 2002. (91)
26. Matrix metalloproteinase-2 is involved in A549 cell migration on laminin-10/11. Gu, J ., Nishiuchi, R., and Sekiguchi, K. Biochem. Biophys. Res. Commun . 296: 69-73, 2002. (8)
27. Csk regulates integrin-mediated signals: involvement of differential activation of ERK and Akt. Gu, J., Nada, S., Okada, M., and Sekiguchi, K. Biochem. Biophys. Res. Commun . 303: 937-977, 2003. (9)
28. Characterization of the ligand binding specificities of integrin alpha3beta1 and alpha6beta1 using a panel of purified laminin isoforms
containing distinct a chains. Nishiuchi, R., Murayama, O., Fujiwara, H., Gu, J., Kawakami, T., Aimoto, S., Wada, Y., and Sekiguchi, K.
J. Biochem. 134:497-504, 2003. (54)
29. Involvement of Oligosaccharide Change in alpha5beta1 Integrin in a Cisplatin-Resistant Human Squamous Cell Carcinoma Cell Line. Nakahara, S., Miyoshi, E., Noda, K., Ihara, S., Gu, J., Honke, K., Inohara, H., Kubo, T., and Taniguchi, N. Mol. Cancer Therap .2:1207-1214, 2003.
30. Relationship between elevated FX expression and increased production of GDP-L-fucose, a common donor substrate for fucosylation in human hepatocellular carcinoma and hepatoma cell lines. Noda, K., Miyoshi, E., Gu, J.,
Gao, C-X., Nakahara, S., Kitada, T., Honke, K., Suzuki, K., Yoshihara, H., Yoshikawa, K., Kawano, K., Tonetti, M., Kasahara, A., H
ori M, Hayashi, N., Taniguchi, N. Cancer Res. 63:6282-6289, 2003.
31. CD151 regulates epithelial cell-cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization. Shigeta, M., Sanzen, N., Ozawa, M., Gu, J., Hasegawa, H., and Sekiguchi, K. J. Cell Biol.163:165-176, 2003. (86)
32. Molecular Cloning and Characterization of Human GnT-IX, A Novel beta1,6- N-Acetylglucosaminyltransferase That Is Specifically Expressed in The Brain. Inamori, K., Endo, T., Ide, Y., Fujii, S., Gu, J., Honke, K., and Taniguchi, N. J. Biol. Chem.
278:43102-43109, 2003.
33. Recombinant Expression and Characterization of a Novel Fibronectin Isoform Expressed in Cartilaginous Tissues. Kozaki, T., Matsui, Y.,
Gu, J., Nishiuchi, R., Sugiura, N., Kimata, K., Ozono, K., Yoshikawa, H. and Sekiguchi, K. J. Biol. Chem. 278:50546-50553, 2003.
34. beta1, 4-N-acetylglucosaminyltransferase III Down-regulates Neurite Outgrowth Induced by Co-stimulation of Epidermal Growth Factor and Integrins Through the Ras/ERK Signaling Pathway in PC12 cells. Gu, J., Zhao, Y., Isaji T., Shibukawa, Y., Ihara, H., Takahashi, M., Ikeda, Y., Miyoshi, E., Honke, K., and Taniguchi, N. Glycobiology 14:177-186, 2004
35. Rac regulates integrin-mediated endothelial cell adhesion and migration on laminin-8. Fujiwara, H., Gu, J., and Sekiguchi, K. Exp. Cell Res. 292:67-77, 2004 (48)
36. N-acetylglucosaminyltransferase IX acts on the GlcNAcbeta 1,2-Manalpha 1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan. Inamori, KI., Endo, T., Gu, J., Matsuo, I., Ito, Y., Fujii, S., Iwasaki, H., Narimatsu, H., Miyoshi, E., Honke, K., and Taniguchi, N. J. Biol. Chem. 279:2337-2340, 2004
37.Introduction of bisecting GlcNAc into integrin alpha5beta1 reduces ligand binding and down-regulates cell adhesion and cell migration . Isaji, T., Gu, J., Nishiuchi, R., Zhao, Y., Takahashi, M., Miyoshi, E., Honke, K., Sekiguchi, K., and Taniguchi, N. J. Biol. Chem. 279:19747-54, 2004. (80)
38. Differential Regulation of Focal Adhesion Kinase and Paxillin Phosphorylation by the Small GTP-binding Protein Rho in Human Corneal Epithelial Cells. Saito, J., Morishige N., Chikama ,T., Gu, J., Sekiguchi,K., Nishida ,T. Jpn J Ophthalmol. 48:199-207, 2004
39. Overexpression of mutated Cu,Zn-SOD in neuroblastoma cells results in cytoskeletal change, Takamiya, R., Takahashi, M., Park, YS., Tawara, Y., Fujiwara, N., Miyamoto, Y., Gu, J., Suzuki, K. and Taniguchi, N. Am. J. Physiol. Cell Physiol . 288, C253-259, 2005.
40. Bisecting GlcNAc Mediates the Binding of Annexin V to Hsp47. Gao, CX, Miyoshi, E., Uozumi ,N., Takamiya ,R., Wang ,X., Noda ,K.,
Gu ,J., Honke, K., Wada, Y., Taniguchi ,N. Glycobiology. 15:1067-75, 2005
41. Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice. Wang, X., Inoue ,S., Gu ,J., Miyoshi, E., Noda, K., Li, W., Mizuno-Horikawa ,Y., Nakano ,M., Asahi, M., Takahashi, M., Uozumi, N., Ihara, S., Lee, SH., Ikeda, Y., Yamaguchi, Y., Aze, Y., Tomiyama, Y., Fujii, J., Suzuki, K., Kondo, A., Shapiro, SD., Lopez-Otin, C., Kuwaki T, Okabe, M., Honke, K., Taniguchi, N. Proc Natl Acad Sci U S A. 102:15791-6, 2005.
42. Core fucosylation regulates EGF receptor-mediated intracellular signaling. Wang X, Gu J, Ihara H, Miyoshi E, Honke K, T aniguchi N. J Biol Chem. 281:2572-7, 2006.
43. Fucosylated haptoglobin is a novel marker for pancreatic cancer: A detailed analysis of the oligosaccharide structure and a possible
mechanism for fucosylation. Okuyama N, Ide Y, Nakano M, Nakagawa T, Yamanaka K, Moriwaki K, Murata K, Ohigashi H, Yokoyama S,
Eguchi H, Ishikawa O, Ito T, Kato M, Kasahara A, Kawano S, Gu J, Taniguchi N, andMiyoshi E. Int. J. Cancer 118:2803-8, 2006.
44. Demonstration of the expression and the enzymatic activity of N-acetylglucosaminyltransferase IX in the mouse brain. Inamori, K., Mita, S., Gu, J., Mizuno-Horikawa, Y., Miyoshi, E., Dennis, JW., and Taniguchi, N. Biochim Biophys Acta 1760:678-84, 2006.
45. High expression of N-acetylglucosaminyltransferase V in favorable neuroblastomas: Involvement of its effect on apoptosis. Inamori, K., Gu, J., Ohira, M., Kawasaki, A., Nakamura, Y., Nakagawa, T., Kondo, A., Miyoshi, E., Nakagawara, A., and Taniguchi, N.
F EBS Letters, 580:627-32, 2006
46. Aberrant expression of N-Acetylglucosaminyltransferase-IVa and IVb ( GnT-IVa and b ) in pancreatic cancer. Ide, Y.,Miyoshi, E., Nakagawa, T., Gu, J., Tanemura, M., Nishida, T., Ito, T., Yamamoto, H., Kozutsumi, Y. and Taniguchi, N. Biochem.
Biophys. Res. Commun . 341:478-82, 2006.
47. Loss of core fucosylation of low-density lipoprotein receptor-related protein-1 impairs its function, leading to the upregulation of serum levels of insulin-like growth factor-binding protein 3 in Fut8-/- mice. Lee, SH., Takahash, M., Honke, K., Miyoshi, E., Osumi, D., Sakiyama, H., Ekuni, A., Wang, X., Inoue, S., Gu, J., Kadomatsu, K., Taniguchi, N. J. Biochem. (Tokyo) 139:391-8, 2006.
48. beta-1,4-N-Acetylglucosaminyltransferase III potentiates {beta}1 integrin-mediated neuritogenesis induced by serum deprivation in Neuro2a cells. Shigeta, M., Shibukawa ,Y., Ihara, H., Miyoshi, E., Taniguchi, N., Gu, J. Glycobiology. 16:564-571, 2006
49. Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells: involvement of E-cadherin-mediated cell adhesion. Iijima, J., Zhao, Y., Isaji, T., Kameyama, A., Nakaya, S., Wang, X., Ihara, H., Cheng, X., Nakagawa, T., Miyoshi, E., Kondo, A., Narimatsu, H., Taniguchi, N., and Gu, J. J. Biol. Chem . 281:13038-46, 2006
50. Down regulation of trypsinogen expression is associated with growth retardation in alpha1,6-fucosyltransferase-deficient mice: attenuation of proteinase-activated receptor 2 activity. Li, W., Nakagawa ,T., Koyama, N., Wang, X., Jin, J., Mizuno-Horikawa ,Y., Gu, J., Miyoshi, E., Kato, I., Honke, K., Taniguchi, N. and Kondo, A. Glycobiology. , 16:1007-19, 2006
51.Fucosylation of N-glycans regulates the secretion of hepatic glycoproteins into bile ducts. Nakagawa, T., Uozumi, N., Nakano, M., Mizuno-Horikawa ,Y., Okuyama, N., Taguchi ,T., Gu, J., Kondo, A., Taniguchi, N., and Miyoshi, E. J. Biol. Chem . 281: 29797-29806, 2006
52. N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha3beta1 integrin-mediated cell migration. Zhao, Y., Nakagawa, T., Itoh, S., Inamori, K. I., Isaji, T., Kariya, Y., Kondo, A., Miyoshi, E., Miyazaki, K.,
Kawasaki, N., Taniguchi, N., and Gu, J. J. Biol. Chem . 281: 32122-32130, 2006
53. N-glycans on the beta-propeller domain of the integrin alpha5 subunit are essential for alpha5beta1 heterodimerization, expression on the cell surface and its biological function. Isaji, T., Sato, Y., Zhao, Y., Miyoshi, E., Wada, Y., Taniguchi, N.,
and Gu, J. J. Biol. Chem . 281: 33258-33267, 2006
54. A secreted type of b1.6 N-acetylglucosaminiyltransferase V (GnT-V), a novel angiogenesis inducer, is regulated by g-secretase. Nakahara S., Saito, T., Kondo, N., Moriwaki, K., Noda, K., Ihara, S., Takahashi, T., Ide, Y., Gu, J., Inohara, H., Katayama, T., T
ohyama, M., Takeshi Kubo, T., Taniguchi, N., and Miyoshi, E. FASEB J. 20:2451-9, 2006
55.Deletion of core fucosylation on alpha3beta1 integrin down-regulates its functions. Zhao, Y., Itoh, S., Wang, X., Isaji, T., Miyoshi, E., Kariya, Y., Kondo, A., Miyazaki, K., Kawasaki, N., Taniguchi, N., and Gu, J. J. Biol. Chem . 281: 38343-38350, 2006
56. Crystal Structure of Mammalian alpha1,6-Fucosyltransferase, FUT8. Ihara, H., Ikeda, Y., Toma, S., Wang, X., Suzuki, T., Gu, J., Miyoshi, E., Tsukihara, T., Honke, K., Matsumoto, A., Nakagawa, A., Taniguchi, N. Glycobiology 17:455-66, 2007
57. Introduction of Bisecting GlcNAc in N-glycan of Adenylyl Cyclase III enhances its activity. Li, W., Takahashi, M., Shibukawa, Y., Yokoe, S., Gu, J., Miiyoshi, E., Ikeda, Y., and Taniguchi, N. Glycobiology 17:655-62, 2007
58. Carbohydrate-binding specificity of a fucose-specific lectin from Aspergillus Oryzae: a novel probe for core fucose. Matsumura, K., Higashida, K., Ishida, H., Hata, Y., Abe, Y., Kato, M., Ueda, M., Yamamoto, K., Shigeta, M., Mizuno, Y., Miyoshi, E., Gu, J. and
Taniguchi, N. J. Biol. Chem .282:15700-8, 2007
59. Human REM-8 is involved in membrane trafficking through early endosomes. Fujibayashi, A., Taguchi, T., Misaki, R., Ohtani, M., Dohmae, N., Takio, K., Yamada, M., Gu, J., Yamakami, M., Fukuda, M., Waguri, S., Uchiyama, Y., Yoshimori, T.
and Sekiguchi, K. Cell Structure Function. 33: 35-50, 2008
60. Biochemical visualization of cell surface molecular clustering in living cells. Kotani, N., Gu, J., Isaji, T., Udaka, K., Taniguchi, N.,
and Honke, K. Proc. Natl. Acad. Sci.105: 7405-9, 2008.
61. N-Acetylglucosaminyltransferase III Expression Is Regulated by Cell-Cell Adhesion via the E-cadherin-catenin-actin Complex. Akama, R., Sato, Y., Kariya, Y., Isaji, T., Fukuda, T., Lu, L., Taniguchi, N., Ozawa, M. and Gu, J. Proteomics 8: 3221–3228, 2008
62. N-glycosylation of laminin-332 regulates its biological functions: A novel function of the bisecting GlcNAc. Kariya, Y., Kato, R.,
Itoh, S., Fukuda, T., Shibukawa , Y., Sanzen, N., Sekiguchi, K., Wada, Y., Kawasaki, N., Gu, J. J. Biol. Chem. 283: 33036-33045, 2008
63. Core fucosylation of E-cadherin enhances cell-cell adhesion in human colon carcinoma WiDr cells. Osumi, D., Takahashi, M., Miyoshi, E., Y okoe, S., Lee, SH., Noda, K., Nakamori, S., Gu, J ., Ikeda, Y., Kuroki, Y., Sengoku, K., Ishikawa, M. and Taniguchi, N. Cancer Sci . 99:1304-1310 , 2009
64. Requirement of Fut8 for the expression of vascular endothelial growth factor receptor-2: a new mechanism for the emphysema-like changes observed in Fut8-deficient mice. Wang , X., Fukuda, T., Li, W., Gao, C., Kondo, A., Matsumoto, A., Miyoshi, E., Taniguchi, N. and Gu, J . J. Biochem . 145:643-51 . 2009
65. N-glycosylation of the I-like domain of beta1 integrin is essential for beta1 integrin expression and biological function: Identification of the minimal N-glycosylation requirement for alpha 5beta 1. Isaji, T., Sato, Y., Fukuda, T. and Gu, J . J. Biol. Chem. 284:12207-16 , 2009
66. An N-glycosylation site on the beta-propeller domain of the integrin alpha5 subunit plays key roles in both its function and site-specific modification by beta1,4-N-acetylglucosaminyltransferase III. Sato, Y., Isaji, T., Tajiri, M., Yoshida-Yamamoto, S., Yoshinaka, T., Somehara, T., Fukuda, T., Wada, Y. and Gu, J . J. Biol. Chem. 284:11873-81 , 2009
67. Bisecting GlcNAc residues on laminin-332 downregulates galectin-3 dependent keratinocyte motility. Kariya, Y., Kawamura, C., Tabei, T., Gu, J . J. Biol. Chem. 285:3330-3340 , 2010
68. Wnt/β-catenin Signaling Down-regulates N-Acetylglucosaminyltransferase III Expression:The implication of two mutually exclusive pathways for regulation. Xu, Q., Akama, R., Isaji, T., Lu, Y., Hashimoto, H., Kariya, Y., Fukuda, T., Du, Y., and
Gu, J. J. Biol. Chem . 286: 4310-8, 2011
69. α1,6-Fucosyltransferase-deficient mice exhibit multiple behavioral abnormalities associated with a schizophrenia-like phenotype: importance of the balance between the dopamine and serotonin systems. Fukuda, T., Hashimoto, H.,
Okayasu, N., Kameyama, A., Onogi, H., Nakagawasai, O, Nakazawa, T., Kurosawa, T., Hao, Y., Isaji, T., Tadano, T.,
Narimatsu , H., Taniguchi, N. and Gu, J . J. Biol. Chem . 286: 18434-43, 2011
70. N-glycosylation of ?4 integrin controls the adhesion and motility of keratinocytes. Kariya, Y. and Gu, J. PLoS One. 6:e27084. 2011
71. Core fucosylation of mu heavy chains regulates the assembly of precursor B cell receptors and its intracellular signaling. Li, W., Liu, Q.,
Pang, Y., Jin, J., Wang, H., Cao, H., Li, Z., Wang, X., Ma, B., Chi, Y., Wang, R., Kondo, A., Gu, J . and Taniguchi, N. J Biol Chem.
287:2500-2508, 2012
72. Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by TGF-β1 in epithelial cell lines. Xu, Q., Isaji, T., Lu, Y., Gu, W., Kondo, M., Fukuda, T., Du, Y. and Gu, J. J Biol Chem. 287:16563-74, 2012
73. Chitosan oligosaccharides suppress production of nitric oxide in lipopolysaccharide- induced N9 murine microglial cells in vitro. Wei, P., Ma, P., Xu, Q., Bai, QH., Gu, J., Xi, H., Du, Y., Yu, C. Glycoconj J. 29:285-95, 2012
74. Anti-tumor effects of exogenous ganglioside GM3 on bladder cancer in an orthotopic cancer model. Wang, H., Isaji, T.,
Satoh, M., Li, D., Arai, Y. and Gu, J. Urology 81, 210.e11-15, 2013
75. E-cadherin and adherens-junctions stability in gastric carcinoma: Functional implications of glycosyltransferases involving N-glycan branching biosynthesis, N-acetylglucosaminyltransferases III and V. Pinho, SS., Figueiredo, J., Cabral, J., Carvalho, S., Dourado, J., Magalh?es, A., G?rtner, F., Mendon?a, AM., Isaji, T., Gu, J., Carneiro, F., Seruca, R., Taniguchi, N., Reis, CA.
Biochim. Biophys. Acta, 1830: 2690-700, 2013
76. a1,6-Fucosylation regulates neurite formation via the activin/phospho-Smad2 pathway in PC12 cells: the implicated dual effects of Fut8 for TGF-b/activin-mediated signaling. Gu, W., Fukuda, T., Isaji, T., Hashimoto, H., Wang., Y and Gu, J. F ASEB J., 27:3947-3958, 2013
77. MicroRNA 130b suppresses migration and invasion of colorectal cancer cells through downregulation of integrin. Zhao, Y.,
Miao, G., Li, Y., Isaji, T., Gu, J., Li, J., Qi, R. PLoS One. 9(2):e87938, 2014
78. An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. Isaji, T., Im, S., Gu, W., Wang, Y., Hang, H., Lu, J., Fukuda, T., Hashii, N., Takakura, D., Kawasaki, N., Miyoshi, H. and Gu, J . J. Biol. Chem. 289: 20694-20705, 2014
79. β-Galactoside α2,6 Sialyltranferase 1 Promotes Transforming Growth Factor-β-Mediated Epithelial-Mesenchymal Transition. Lu, J., Isaji, T., Im, S., Fukuda, T., Hashii, N., Takakura, D., Kawasaki, N. and Gu, J. J. Biol. Chem. 289: 34627-34641, 2014
80. Loss of α1,6-fucosyltransferase Suppressed Liver Regeneration: Implication of Core Fucose In The Regulation of Growth Factor Receptor-mediated Cellular Signaling. Wang, Y., Fukuda, T., Isaji, T., Lu, J., Gu, W., Ohkubo, Y., Kamada, Y., Taniguchi, N., Miyoshi, E. and Gu, J. Sci. Rep. 5, 8264; DOI: 10.1038/srep08264, 2015
81. Core fucosylation of IgG-BCR is required for antigen recognition and antibody production. Li, W., Ma, B.,(他8名)Fukuda, T., Gu, J., Zhang, J. and Taniguchi, N. J. Immuno . 194, 2596-2606, 2015
82. Loss of α1,6-fucosyltransferase inhibits chemical-induced hepatocellular carcinoma and tumorigenesis by down-regulating multiple cellular signaling. Wang, Y., Fukuda, T., Isaji, T., Lu, J., Im, S., Hang, Q., Gu, W., Hou, S., Ohtsubo, K.
and Gu, J. FASEB J., in press
83. Loss of α1,6-fucosyltransferase decreased hippocampal long-term potentiation:implications for core fucosylation in the regulation of AMPA receptor heteromerization and cellular signaling.Gu, W.,Fukuda, T., Isaji, T., Hang, Q., Lee, H., Sakai, S.,
Morise, J., Mitoma, J., Higashi, H.,Taniguchi, N., Yawo, H., Oka, S. and Gu, J. J. Biol. Chem. In press