硕士生导师

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姚文娟

作者:金飞     日期:2021-10-26   点击数:2554  

师资介绍


姚文娟,女,汉族,198304月生,博士,教授,硕士生导师。



教育经历:

2000.09-2004.06南京师范大学,生物学,学士

2004.09-2007.06南京师范大学,生物化学与分子生物学,硕士

2007.09-2010.06中国药科大学,微生物与生化药学,博士



工作经历:

2010.07-至今  南通大学,药学院,教授

2017.07-2018.07 澳大利亚悉尼大学,药学院,访问学者(江苏省公派)



研究方向:

心血管药理毒理



基金项目:

  1. 国家自然科学基金面上项目,81970226SUMO化修饰调节RhoGDI蛋白降解在AngІІ介导的VSMC表型转化和动脉血管重构中的作用及机制研究,2020/01-2023/1255万元,结题,主持。

  2. 国家自然科学基金青年项目,81202879,何首乌活性成分二苯乙烯苷对波形蛋白骨架系统的调控及在防治动脉粥样硬化中的作用,2013/01-2015/1223万元,结题,主持。

  3. 南通市自然科学基金,JC2023041,锚定于trans-Golgi网络的RhoGDI3调控NLRP3炎症小体装配、活化在血管新生内膜增生中的作用及机制研究,2023/09-2026/0815万元,在研,主持。

  4. 江苏省高校自然科学研究重大项目,19KJA320010RhoGDI降解在平滑肌细胞转分化及血管重构中的作用及机制, 2019/09-2022/0815万元,结题,主持。

  5. 南通市科技计划项目,JC2018059Rho通路对波形蛋白的调控在内皮细胞凋亡中的作用及机制研究,2018/07-2020/063万元,结题,主持。

  6. 江苏省高校自然科学基金面上项目,16KJB360007Rho/ROCK调控波形蛋白骨架及二苯乙烯苷的干预,2016/09-2018/083万元,结题,主持。



科研成果:

近几年一直致力于研究血管稳态(动脉硬化、重构)的分子机制及发现新型分子药靶。课题组通过蛋白组学方法在动脉粥样硬化大鼠主动脉组织中发现Vimentin表达及裂解具有重要作用,并发现TGF-β/Smad信号通路和Caspase3活化调控Vimentin表达及裂解,从而参与TNF-αox-LDL诱导的内皮细胞损伤、凋亡;ICAM-1VCAM-1表达以及它们与Vimentin的共定位参与了内皮细胞与单核细胞的黏附。同时,还筛选出二苯乙烯苷TSG可通过抑制Vimentin表达和骨架形态,抑制TNF-α诱导的VSMCs迁移和ox-LDL诱导的泡沫细胞形成及巨噬细胞凋亡;TSG还可抑制ox-LDL诱导的单核细胞中ICAM-1表达及其与Vimentin的共定位。这些结果为波形蛋白作为新的抗动脉粥样硬化作用靶分子提供依据,也为抗AS创新药物研发奠定基础。近期还发现RhoGDI的蛋白降解减少引起其病理性积聚参与了AngІІ介导的VSMC增殖,即RhoGDI可能参与动脉粥样硬化性血管病变;在小鼠AngІІ缓释模型中,RhoGDI的蛋白水平显著上升,knockdown下调RhoGDI蛋白水平后可有效抑制血管重构;AngІІ介导的VSMC增殖和RhoGDI蛋白病理性积聚可被SUMO化抑制剂阻断,即SUMO化修饰可能参与调控RhoGDI的蛋白降解及AngІІ诱导的VSMC增殖;并且RhoGDISUMO化修饰和泛素化修饰存在竞争性结合作用,总之,发现了一种引起VSMC表型转化及血管重构的新机制。

科研成果受到2项国家自然科学基金项目,1项江苏省高校自然科学研究重大项目,1项江苏省高校自然科学基金面上项目,多项南通市科技计划项目的支持。并发表SCI论文数篇,其中一些发表于认可度较好的SCI杂志,例如Atherosclerosis, Pharmacological Research, Biomedicine and pharmacotherapy, Toxicology等。获得授权发明专利三项。



代表性论文:

  1. Sun J, Shao Y, Pei L, Zhu Q, Yu X,Yao W通讯作者. AKAP1 alleviates VSMC phenotypic modulation and neointima formation by inhibiting Drp1-dependent mitochondrial fission. Biomed Pharmacother. 2024, 176:116858.

  2. Sun J, Zhu Q, Yu X, Liang X, Guan H, Zhao H,Yao W通讯作者.RhoGDI3 at the trans-Golgi network participates in NLRP3 inflammasome activation, VSMC phenotypic modulation, and neointima formation. Atherosclerosis, 2023, 387: 117391.

  3. Guan H, Sun J, Liang X,Yao W(通讯作者). Protective role of Cytochrome C Oxidase 5A (COX5A) against mitochondrial disorder and oxidative stress in VSMC phenotypic modulation and neointima formation. Curr Vasc Pharmacol, 2023, 21(2): 128-142.

  4. Liang X, Sun J, Guan H, Zhu Q,Yao W(通讯作者). Angiotensin II inhibits adipogenic differentiation and promotes mature adipocyte browning through the corepressor CtBP1. Biomedicines. 2022, 10(12): 3131.

  5. Qi Yan, Liang Xiuying, Guan Haijing, Sun Jingwen,Yao Wenjuan通讯作者. RhoGDI1-Cdc42 signaling is required for PDGF-BB-induced phenotypic transformation of vascular smooth muscle cells and neointima formation.Biomedicines, 2021, 9(9): 1169.

  6. Liang Xiuying, Guan Haijing, Sun Jingwen, Qi Yan,Yao Wenjuan(通讯作者). Comparative proteomic analysis of tPVAT during Ang II infusion. Biomedicines, 2021, 9: 1820.

  7. Qi Yan, Guan Haijing, Liang Xiuying, Sun Jingwen, Yao Wenjuan(通讯作者). Ang II promotes SUMO2/3 modification of RhoGDI1 through Aos1 and Uba2 subunits, and then regulates RhoGDI1 stability and cell proliferation. Cardiovasc. Drugs. Ther., 2021, 35(4): 769-773.

  8. Qi Yan, Liang Xiuying, Dai Fan, Guan Haijing, Sun Jingwen,Yao Wenjuan(通讯作者). RhoA/ROCK pathway activation is regulated by AT1 receptor and participates in smooth muscle migration and dedifferentiation via promoting actin cytoskeleton polymerization. Int. J. Mol. Sci., 2020, 21(15): 5398.

  9. Xiuying Liang, Yan Qi, Fan Dai, Jingya Gu,Wenjuan Yao(通讯作者). PVAT: an important guardian of the cardiovascular system. Histol. Histopathol., 2020, 35 (8): 779-787.

  10. Fan Dai, Yan Qi, Wei Guan, Guoliang Meng, Zhaoguo Liu, Ting Zhang,Wenjuan Yao(通讯作者). RhoGDI stability is regulated by SUMOylation and ubiquitination via the AT1 receptor and participates in Ang II-induced smooth muscle proliferation and vascular remodeling. Atherosclerosis 288 (2019) 124–136.

  11. Lian Tang , Fan Dai, Yan Liu, Xiaoqiang Yu, Chao Huang, Yuqin Wang,Wenjuan Yao(通讯作者). RhoA/ROCK signaling regulates smooth muscle phenotypic modulation and vascular remodeling via the JNK pathway and vimentin cytoskeleton. Pharmacological Research, 2018, 133: 201–212.

  12. Lifeng Yang, Lian Tang, Fan Dai, Guoliang Meng, Runting Yin, Xiaole Xu,Wenjuan Yao(通讯作者. Raf-1/CK2 and RhoA/ROCK signaling promote TNF-α-mediated endothelial apoptosis via regulating vimentin cytoskeleton. Toxicology, 2017, 389: 74–84.

  13. Wenjuan Yao(第一作者兼通讯作者), Huang Lei, Qinju Sun, Lifeng Yang, Lian Tang, Guoliang Meng, Xiaole Xu, Wei Zhang. The inhibition of macrophage foam cell formation by tetrahydroxystilbene glucoside is driven by suppressing vimentin cytoskeleton. Biomedicine & Pharmacotherapy, 2016, 83: 1132-1140.IF=4.545,二区)

  14. Wenjuan Yao(第一作者兼通讯作者), Chengjing Gu, Haoran Shao, Guoliang Meng, Huiming Wang, Xiang Jing, Wei Zhang. Tetrahydroxy stilbene glucoside improves TNF-α-induced endothelial dysfunction: involvement of TGFβ/Smad pathway, and inhibition of vimentin expression. The American Journal of Chinese Medicine, 2015, 43 (1): 183-198.

  15. Yao WenJuan(第一作者)Fan WenJunHuang ChaoZhong HuiChen XiangFan, Zhang Wei. Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats. Biomedicine & Pharmacotherapy, 2013, 67 (2): 140-145.



授权发明专利:

南通大学.银杏酸在制备和治疗血管再狭窄疾病药物中的应用.姚文娟,梁锈瑛,祁妍,陈云,刘兆国. ZL202010722910.6.  2022-8-12.

南通大学.携带人源RhoGDI3基因shRNA腺病毒的构建及其应用.姚文娟,梁锈瑛,祁妍,戴凡. ZL202010498909.X.  2022-12-02.

南通大学.生产聚谷氨酸的重组谷氨酸棒杆菌及构建方法和用途.姚文娟,孟国梁,张伟,陈向凡,殷润婷,ZL201310079151.6 2014-10-22