于艳艳

师资介绍

于艳艳，女，汉族，1988年9月生，博士，副教授，硕士生导师。江苏省优秀青年基金项目获得者。

教育经历：

2006.09-2010.06吉林大学，药学，学士

2010.09-2015.06中山大学，药物分析学，博士

工作经历：

2015.09-2020.07南通大学，药学院，讲师

2016.10-2018.10中科院上海应物所，博士后

2018.11-2019.11美国佐治亚理工学院，访问学者

2020.07-至今 南通大学，药学院，副教授

研究方向：

药物分析学，分子诊断

基金项目：

1. 南通市科技局，科技计划项目，JC2025026，加权线性等温扩增机制介导的Tau蛋白病理评估分子分类器研究，2025-07至2028-06，14万元，在研，主持

2. 江苏省科技厅，优秀青年基金，BK20211604，基于CRISPR技术的免疫层析试纸法用于SARS-CoV-2双靶标联合检测，2021-07至2024-06，50万元，已结题，主持

3. 南京大学“生命分析化学国家重点实验室” 开放基金，SKLACLS2013，变构响应型多信号三维DNA探针的构建及应用，2020-04至2022-03，5万元，已结题，主持

4. 南通市科技局，社会民生面上，MS12021051，CRISPR多通道响应新技术的开发及用于SARS-CoV-2可视化现场检测，2021-07至2023-06，10万元，已结题，主持

5. 南通市科技局，新型冠状病毒专项，XG202008-4，等温扩增式3D折纸芯片的研发及用于新型冠状病毒检测，2020-03至2022-02，4万元，已结题，主持

6. 国家自然科学基金委员会，青年项目，21705082，纸基变构响应型三维传感界面的构建及其p53/MDM2相互作用抑制剂研究应用，2018-01至2020-12，25万元，已结题，主持

7. 江苏省科技厅，青年项目，BK20170444，3D折纸芯片传感系统的构建及用于p53/MDM2相互作用抑制剂的研究，2017-07至2020-06，20万元，已结题，主持

8. 中国博士后科学基金会，面上项目，2017M611640，集成式双信号响应型三维纳米探针的构建及传感应用，2017-01至2018-11，5万元，已结题，主持

9. 南通市科技局，科技计划项目，MS12016023，基于DNA等温指数扩增的生物传感器用于卵巢癌的早期联合诊断，2016-10至2018-09，3万元，已结题，主持

科研成果：

主要从事基于DNA纳米技术的药物分析和分子诊断新方法的研究，近年来针对药学研究和临床应用的发展需求，致力于高性能DNA传感新技术的开发及其生物医学应用。围绕DNA传感技术的关键科学问题，从响应新策略研究、纳米探针设计和应用体系开发等方面逐步深入，在临床辅助诊断，疾病标志物分析领域取得了系列创新性学术成果。

截至目前，以第一/通讯作者在Analytical Chemistry、Biosensors and Bioelectronics、Sensors and Actuators: B. Chemical、Chemical Communications等期刊发表SCI论文20余篇，其中中科院一区论文14篇，二区论文10篇，自然指数期刊论文9篇，ESI高被引论文2篇，2篇论文被封面报道，总被引2000余次，H-index为24。

研究工作受到国家自然科学基金委员会、江苏省科技厅和南通市科技局各类基金项目支持，包括国家自然科学青年科学基金1项、江苏省自然科学青年基金和优秀青年基金各1项。

成果奖励：

1、江苏省高等学校科学技术研究成果奖，基于CRISPR-Cas技术的分子诊断研究与应用，2023年，三等奖（第一完成人）。

部分代表性论文：

1. Yanan Lin,Dawei Jiang,Xingpeng Dong,Yilin Li,Xiyue Wu,Ruoxuan Li,Feng Li, Dan Sun*,Yanyan Yu*,A label-free orthogonal dual-channel CRISPR-Casplatform for simultaneous detection ofMycobacterium tuberculosis and respiratorysyncytial virus, Chemical Communications, 2025, 61, 17914-17917

2. Yan Zhang,Yanan Lin,Ruoxuan Li,Dawei Jiang,Ruiyang Cai,Gaoxing Su*,Yanyan Yu*,Guangbo Qu,Split G-Quadruplex-Integrated CRISPR-Cas Biosensor for One-Pot,Signal-On Visual Detection of Pathogen Nucleic Acids,Analytical Chemistry, 2025, 97, 13577–13585

3. Yilin Li#,, Feng Li#,, Ruoxuan Li, Yuedong Zhu,, Yanan Lin,Yan Zhang, Dan Sun*,Yanyan Yu*,Single nucleotide polymorphism discrimination and genotyping based oncascade strand displacement reaction mediated label-free Cas12a system,Sensors and Actuators: B. Chemical, 2025, 423, 136832

4. Yuedong Zhu#, Yanan Lin#, Bin Gong , Yan Zhang , Gaoxing Su*,Yanyan Yu*，Dual toeholds regulated CRISPR-Cas12a sensing platform for ApoE single nucleotide polymorphisms genotyping，Biosensors and Bioelectronics，2024, 255, 116225

5. Gaoxing Su, Mengting Xu, Yuedong Zhu, Yan Zhang, Yanan Lin,Yanyan Yu*Simultaneous and multiplexed phenotyping of circulating exosomes with the orthogonal CRISPR–Cas platform，Chemical Communications, 2024, 60, 5944

6. Yan Zhang#, Bin Gong#, Yanan Lin, Yuedong Zhu, Gaoxing Su*,Yanyan Yu*, Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping,Analyst, 2024, 149, 707

7. Shujing Ren, Yuhang Xu, Xingpeng Dong, Qingxin Mu, Xia Chen*,Yanyan Yu*, Gaoxing Su*, Nanotechnology-empowered combination therapy for rheumatoid arthritis: principles, strategies, and challenges, Journal of Nanobiotechnology, 2024, 22, 431

8. Yuliang Kang#, Jiali Wang#, Wensi Zhang, Yuhang Xu, Bohui Xu, Guangbo Qu,Yanyan Yu*, Bing Yan*, Gaoxing Su*, RNA extraction-free workflow integrated with a single-tube CRISPR-Cas-based colorimetric assay for rapid SARS-CoV-2 detection in different environmental matrices，Journal of Hazardous Materials, 2023, 454,131487

9. Mengting Xu#, Yuedong Zhu#, Yan Zhang, Bin Gong, Yuliang Kang, Gaoxing Su *，Yanyan Yu*，Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system，Chemical Communications，2023, 59, 7615-7618

10. Yuliang Kang, Gaoxing Su*，Yanyan Yu*，Jiajia Cao, Jiali Wang, Bing Yan*，CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater，Environmental Science & Technology，2022, 56, 4101−4110

11. JialongWu,Yanyan Yu* and Gaoxing Su*，Safety Assessment of 2D MXenes: In Vitro and In Vivo，Nanomaterials，2022, 12, 828

12. Gaoxing Su#, Min Zhu#, Diyuan Li, Mengting Xu, Yuedong Zhu, Yan Zhang, Hongyan Zhu, Feng Li*,Yanyan Yu*，Multiplexed lateral flow assay integrated with orthogonal CRISPR-Cas system for SARS-CoV-2 detection，Sensors and Actuators: B. Chemical，2022，371，132537

13. Min Zhu#, Jianbin Pan#, Mengting Xu, Jiajia Cao, Yuedong Zhu, Hongyan Zhu, Gaoxing Su*,Yanyan Yu*，P53-MDM2 interaction monitoring and inhibitors potency evaluation based on CRISPR-Cas12a sensing platform，Sensors and Actuators: B. Chemical，2022，361，131710

14. Wei S. Zhang#，Jianbin Pan#, Feng Li, Min Zhu, Mengting Xu, Hongyan Zhu,Yanyan Yu*, Gaoxing Su*, Reverse-Transcription Recombinase Polymerase Amplification Coupled CRISPR-Cas12a for Facile and Highly Sensitive Colorimetric SARS-CoV-2 Detection, Analytical Chemistry,2021, 93 (8): 4126–41333

15. Yanyan Yu#, Wei S. Zhang#, Yuehua Guo, Huaping Peng, Min Zhu, Dandan Miao, Gaoxing Su*, Engineering of exosome-triggered enzyme-powered DNA motors for highly sensitive fluorescence detection of tumor-derived exosomes,Biosensors and Bioelectronics,2020, 167: 112482

16. Yanyan Yu#, Zhongnan Huang#, Yao Zhou, Lihua Zhang, Ailin Liu, Wei Chen*, Jianhua Lin*, Huaping Peng*, Facile and highly sensitive photoelectrochemical biosensing platform based on hierarchical architectured polydopamine/tungsten oxide nanocomposite film,Biosensors and Bioelectronics,2019, 126: 1-6

17. Gaoxing Su#, Min Zhu#, Mengting Xu, Jianbin Pan, Yao Zhou, Hongyu Zhou, Wei S. Zhang, Yanli Tong*,Yanyan Yu*, Fluorescence resonance energy transfer-based DNA framework assembled split G-quadruplex nanodevices for microRNA sensing,Chemical Communications,2020, 56 (88): 13583-13586

18. Min Zhu,Yanyan Yu*, Junfeng Zhu, Yao Zhou, Gaoxing Su, Hongyan Zhu, Yong Chen, Mingkai Liu*, Single nucleotide variant discrimination by toehold exchange spherical nucleic acids modulated on hierarchical molybdenum disulfide acanthospheres,Chemical Communications,2020, 56 (61): 8599-8602

19. Dandan Miao,Yanyan Yu*, Yong Chen, Yin Liu, Gaoxing Su*, Facile Construction of i-Motif DNA-Conjugated Gold Nanostars as Near-Infrared and pH Dual-Responsive Targeted Drug Delivery Systems for Combined Cancer Therapy,Molecular Pharmaceutics,2020, 17 (4): 1127-1138

20. Yanyan Yu, Yao Zhou, Min Zhu, Gaoxing Su*, Haohua Deng, Wei Chen*, Huaping Peng*, Dynamic split G-quadruplex programmed reversible nanodevice,Chemical Communications,2019, 55 (3): 389-392

21. Yanyan Yu, Yao Zhou, Min Zhu, Mingkai Liu, Hongyan Zhu, Yong Chen, Gaoxing Su*, Wei Chen*, Huaping Peng*, Programming a split G-quadruplex in a DNA nanocage and its microRNA imaging in live cells,Chemical Communications,2019, 55 (35): 5131-5134

22. Yanyan Yu#, Min Zhou#, Wei Zhang, Lei Huang, Dandan Miao, Hongyan Zhu*, Gaoxing Su*, Rattle-Type Gold Nanorods/Porous-SiO2 Nanocomposites as Near-Infrared Light-Activated Drug Delivery Systems for Cancer Combined Chemo–Photothermal Therapy,Molecular Pharmaceutics,2019, 16 (5): 1929-1938

23. Yu Yanyan, Su Gaoxing, Zhu Hongyan, Zhu Qing, Chen Yong, Xu Bohui, Li Yuqin, Zhang Wei*. Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection,International Journal of Nanomedicine,2017,12: 5903-5914

24. Yanyan Yu, Zuanguang Chen*, Wensi Jian, Duanping Sun, Beibei Zhang, Xinchun Li, Meicun Yao*, Ultrasensitive electrochemical detection of avian influenza A (H7N9) virus DNA based on isothermal exponential amplification coupled with hybridization chain reaction of DNAzyme nanowires,Biosensors and Bioelectronics, 2015, 64: 566-571

25. Yanyan Yu, Zuanguang Chen*, Lijuan Shi, Fan Yang, Jianbin Pan, Beibei Zhang, Duanping Sun, Ultrasensitive Electrochemical Detection of MicroRNA Based on an Arched Probe Mediated Isothermal Exponential Amplification,Analytical Chemistry, 2014, 86 (16): 8200-8205

26. Yanyan Yu, Zuanguang Chen, Sijing He, Beibei Zhang, Xinchun Li, Meicun Yao*, Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode,Biosensors and Bioelectronics, 2014, 52: 147-152

27. Yanyan Yu, Zuanguang Chen*, Beibei Zhang, Xinchun Li, Jianbin Pan, Selective and sensitive determination of uric acid in the presence of ascorbic acid and dopamine by PDDA functionalized graphene/graphite composite electrode,Talanta, 2013, 112: 31-36

授权专利：

（1）于艳艳;朱敏;苏高星;徐梦婷;朱越东;基于CRISPR-Cas12a传感系统的PPIs检测平台及检测方法,2021-11-19,中国, 202111399079.6

（2）于艳艳;朱敏;苏高星;一种DNA框架包裹的G-四链体结构及其制备方法与应用, 2025-01-03,中国, ZL202011071468.1

（3）于艳艳;朱敏;周垚;苏高星;朱红艳;一种荧光共振能量转移生物传感器及其应用, 2021-04-27,中国, ZL201810829706.7